Comprehensive analysis of RNA binding proteins in the immune system

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H06898
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Kyoto University
• Principal Investigator: Uehata Takuya 京都大学, ウイルス・再生医科学研究所, 助教 (50785970)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: RNA結合蛋白質 / 転写後制御 / 炎症制御

Outline of Final Research Achievements

RNA binding proteins (RBPs) play a central role in the fate decision of mRNA. Better understanding of dynamic changes in RBP-RNA interaction during inflammation is important for discovery of new post-transcriptional mechanisms. In this study, RBP-RNA interactome was determined using T cell line post-activation. I found that 750 RBPs were detected and that a gene cluster related to inflammatory responses was identified from among them. RBPs that are presumably involved in inflammatory responses were selected and I obtained preliminary data that these RBPs regulate inflammation including cytokine production. Collectively, I demonstrated that RBPs underwent a dynamic change in RNA interaction status during inflammation.

Free Research Field

免疫学