Generation of myogenic stem cells from iPS cells via blastocyst complementation.

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H07001
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Neurology
• Research Institution: Hiroshima University
• Principal Investigator: Ito Hikaru 広島大学, 医歯薬保健学研究科(医), 助教 (50587392)
• Research Collaborator: Asakura Atsushi University of Minnesota, Department of Neurology, Stem Cell Institute, Associate Professor ; Asakura Yoko University of Minnesota, Department of Neurology, Stem Cell Institute, Technical staff(Researcher2)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: 再生医療 / 骨格筋幹細胞 / 胚盤胞胚補完法 / 発生工学 / 筋ジストロフィー / サルコペニア

Outline of Final Research Achievements

One attractive therapy is myogenic stem cell transplantation to provide dystrophin in Duchenne Muscular Dystrophy (DMD) patients. Recent work has reported the induced pluripotent stem cell (iPSC)-derived myogenic stem cell transplantation for therapeutic strategy for DMD. However, there is a limitation in this strategy, especially in low differentiation efficiency and poor reproducibility of iPSCs-derived myogenic stem cells. Therefore, in a blastocyst complementation method, the regeneration of an organ can be achieved by utilizing the defect in an organ which can be complemented by injecting patient-derived iPSCs into a non-human blastocyst.
In this study, we injected GFP (+) mouse iPSCs into skeletal muscle-less mouse blastocysts. iPSC-derived cells highly contributed to limb muscle of chimera. We also performed intramuscular injection of myogenic stem cells isolated from chimeric mice into mdx mice, and detected GFP (+) muscle fibers which also expressed donor-derived dystrophin.

Free Research Field

再生医療