The mechanism of TMJ-OA pathogenesis in HIF-1a gene deficient mice

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H07019
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: MORI Hiroki 徳島大学, 大学院医歯薬学研究部(歯学系), 助教 (90779985)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: TMJ

Outline of Final Research Achievements

Mechanical stress was applied to the TMJ of C57BL/6NCr wild-type (WT) and HIF-1α+/－ mice with a sliding plate for 10 days. Histological analysis was performed by HE staining, Safranin-O/Fast green staining, and immunostaining specific for articular cartilage homeostasis.HIF-1α+/－ mice had thinner cartilage and smaller areas of proteoglycan than WT controls, without and with mechanical stress. Mechanical stress resulted in prominent degenerative changes with increased expression of HIF-1α, VEGF, and the apoptosis factor cleaved Caspase-3 in condylar cartilage. Our results indicate that HIF-1α may be important for articular cartilage homeostasis and protective against articular cartilage degradation in the TMJ under mechanical stress condition, therefore HIF-1α could be an important new therapeutic target in TMJ-OA.

Free Research Field

矯正歯科