Factors of radioresistance for oral cancer cells which were treated with KPU-300 radiosensitizing therapy.

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H07076
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Nagasaki University
• Principal Investigator: OKUYAMA Kohei 長崎大学, 病院(歯学系), 助教 (30781968)
• Research Collaborator: SUZUKI Keiji 長崎大学, 原爆後障害医療研究所, 准教授 (00196809)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: Fucci / 舌癌 / 細胞周期 / 放射線増感 / 微小管重合阻害 / M期 / KPU-300

Outline of Final Research Achievements

KPU-300 is a novel colchicine-type anti-microtubule agent derived from pulinabulin (NPI-2358). We characterized the effects of KPU-300 on cell cycle kinetics and radiosensitization using SAS cells, oral tongue cancer cell line, expressing the fluorescent ubiquitination-based cell cycle indicator (Fucci). KPU-300 was reported as a potent radiosensitizer which is due to the synchronization of cells in M phase. However, SAS-Fucci cells did not do that; cells tend to be arrested in G1 phase and dead cells were gathered with the center of colony.

Free Research Field

口腔がん