Recycling of insulin by pH

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H07083
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Metabolomics
• Research Institution: Oita University
• Principal Investigator: Yamaoka Mami 大分大学, 医学部, 特任助教 (10783847)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: 糖尿病 / インスリン / エンドサイトーシス / 膵B細胞 / エキソサイトーシス / 膜輸送 / シグナル伝達 / Gタンパク質

Outline of Final Research Achievements

Glucose promotes membrane fusion between insulin secretory membranes and plasma membranes, and thereby releases insulin from the vesicles in pancreatic beta-cells. Glucose also promotes endocytosis of the fused secretory membranes. Although endocytosis is essential for cell size maintenance before and after insulin secretion and for refilling of insulin granule pools, little is known about the underlying molecular mechanisms. In the present study, I found that Rab27a regulates the complex formation of proton pumps. These results suggest that Rab27a regulates endocytosis via acidification in endocytosed vesicles.

Free Research Field

分子糖尿病学