2017 Fiscal Year Final Research Report
Identification of host factors cleaved by HIV protease for elucidating the mechanism of innate immune evasion.
| Project/Area Number |
16H07105
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Keywords | HIV / プロテアーゼ / 自然免疫 / 細胞死 |
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| Outline of Final Research Achievements |
In this study, we investigated the cleavage of host factors related to innate immune response by HIV protease (HIV-PR). We identified 4 host substrates being cleaved by HIV-PR using in in vitro assay system with recombinant proteins. Among them, we selected CASP1 since it is a key factor for pyroptotic cell death. We confirmed that HIV protease cleaved CASP1 in cells leading to the secretion of intracellular LDH to extracellular space due to the cell membrane damage. We also found that a protease inhibitor DRV specifically suppressed the CASP1 cleavage and resultant cell toxicity by HIV-PR. These results indicate a possibility that HIV-1 protease is involved in the pyroptotic cell death mediated CASP1.
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| Free Research Field |
タンパク質工学、ウイルス学
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