Tolerance induction by CD8+/CD122+ Regulatory T Cells in Organ Transplantation

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H07121
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Nakamura Tsukasa 京都府立医科大学, 医学(系)研究科(研究院), 助教 (30777959)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: 制御性T細胞 / 骨髄由来抑制細胞 / 臓器移植

Outline of Final Research Achievements

Background: Myeloid-derived suppressor cells (MDSCs) maintain host immunity: regulating rejection. On the other hand, CD8+ Tregs also play a key role in preventing rejection. However, the relationship between MDSCs and CD8+ Tregs is unclear. Here, the results revealed that MDSCs have a potential to recruit CD8+/IL-10+ Tregs. Methods: MDSCs were isolated from bone-marrow culture with GM-CSF, M-CSF, and dexamethasone (MDSCs-Dex). In murine heterotopic cardiac transplantation, MDSCs were transferred through the tail vein. Results: Flow cytometric analyses showed that MDSCs-Dex led to significant recruitment of CD8+/IL-10+ Tregs (3.3±2.1% vs 10.2±3.6%, p<0.05). In a MDSCs transfer model, pathological findings also confirmed accumulation of CD8+/PD-1+ Tregs in an allograft. Conclusion:MDSCs might result in recruitment of CD8+/IL-10+ Tregs. These results suggested that the synergistic effect between MDSCs and CD8+ Tregs developed a tendency to immunological tolerance.

Free Research Field

臓器移植