2017 Fiscal Year Final Research Report
Establishment of multi-dimensional analysis for monitoring pathophysiological status of pregnancy-induced hypertension
| Project/Area Number |
16H07187
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Keywords | 妊娠高血圧症候群 / 疾患バイオマーカー / 多元的病態評価 / ターゲットプロテオミクス |
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| Outline of Final Research Achievements |
We previously developed potential serum disease biomarkers (DBMs) of hypertensive disorder of pregnancy (HDP). Our methodology did not extend to PDA071 (cysteinyl α2-HS-glycoprotein341-367), due to difficulty to produce a specific antibody against the peptide. The aim of the present study was to establish an alternative PDA071 quantitation system using LC-MRM/MS, to explore the potential utility of PDA071 as a DBM for HDP. PDA071 was successfully extracted from serum using a heat denaturation method. Optimum conditions for quantitation via LC-MRM/MS were developed. Although the PDA071 alone did not significantly differ between patients and controls, 3D plotting of PDA039/044/071 peptide levels and construction of a Jackknife classification matrix were satisfactory in terms of HDP diagnostic precision. Combination analysis using both PDA071 and PDA039/044 levels allowed HDP diagnostic accuracy to be attained, and our method will be valuable in future pathophysiological studies of HDP.
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| Free Research Field |
産科婦人科学
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