The roles of ILC2 and basophils on the severity of allergic conjunctivitis

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H07189
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Juntendo University
• Principal Investigator: Jobu Sugita 順天堂大学, 医学部, 助手 (90622712)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: アレルギー性結膜炎 / 好塩基球 / 好酸球 / ILC2 / 2型炎症 / サイトカイン

Outline of Final Research Achievements

We found basophil depletion in mouse papain-induced allergic conjunctivitis model induced significant suppression of eosinophil infiltration. We then found that innate lymphoid cell type2 (ILC2) depletion in mouse papain-induced allergic conjunctivitis model induced significant suppression of eosinophil infiltration. Furthermore, we found activation of ILC2 in conjunctival tissue was suppressed in allergic conjunctivitis model using IL-33 (one of the signature cytokines of type 2 immune reaction) knockout mouse. In addition, we found the existence of ILC2 in severe chronic human allergic conjunctivitis tissue (giant papillae tissue obtained from vernal keratoconjunctivitis patients) using flow cytometer (FACS).

Free Research Field

眼科学