Elucidation of the role of NADPH oxidase in cartilage degenerative diseases

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H07195
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Showa University
• Principal Investigator: INOUE SAKIE 昭和大学, 歯学部, 助教 (20783252)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: 軟骨 / 軟骨変性疾患 / NADPHオキシダーゼ

Outline of Final Research Achievements

In this study, we found that NADPH oxidase (NOX)-2 activity-dependent acidification induced activation of hyaluronidase in chondrocyte cultures, which caused reduction in extracellular matrix. In rheumatoid arthritis, bone resorption progresses in addition to cartilage degradation. Hence, we studied the molecular mechanism of bone resorption occurs in an NO- and ROS-dependent manner. We found that 8-nitro-cGMP, a novel downstream signaling molecule of NO and ROS, was produced by osteoclasts and its progenitor cells in an NADPH oxidase-dependent manner after stimulation by inflammatory cytokines. We also found that 8-nitro-cGMP promoted osteoclast differentiation.

Free Research Field

軟骨変性疾患