2017 Fiscal Year Final Research Report
The study of the synaptonemal complex component SYCP3 in meiotic recombination
| Project/Area Number |
16H07295
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Genome biology
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Keywords | 相同組換え / シナプトネマ複合体 / SYCP3 / RAD51 / DMC1 / 相同的対合 |
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| Outline of Final Research Achievements |
The purpose of this study is to elucidate the molecular mechanism of meiotic recombination by the synpatonemal complex component SYCP3. The synaptonemal complex is essential for the progression of meiotic recombination. However, the functions of SYCP3 in meiotic recombination remain elusive. We performed the biochemical and cell biology analyses to elucidate the mechanism by which SYCP3 regulates RAD51- and DMC1- homologous pairing. We found that SYCP3 significantly suppresses the RAD51-mediated homologous pairing, but not DMC1-mediated homologous pairing. In addition, we found that SYCP3 specifically binds to RAD51 and suppresses RAD51-mediated homologous pairing by competing with HOP2-MND1 complex.
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| Free Research Field |
生化学 構造生物学
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