2017 Fiscal Year Final Research Report
Investigation of chlamydial pathological mechanism by using establishment of a system for expressing chlamydial type III effector proteins
| Project/Area Number |
16H07312
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Tokai Gakuin University |
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Principal Investigator |
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Keywords | Chlamydia / Parachlamydia |
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| Outline of Final Research Achievements |
Usual chlamydial protein expression system is expressing in E. coli, but it is not very efficiently. So, I challenged to establishing of an expression system by using Brevibacillus Expression System II.
Parachlamydia acanthamoeba was used in the experiment, it is easy to handling and closely related species for Chlamydia trachomatis that is the serious issue of a cause of sexually transmitted disease. Consequently, chlamydial proteins was not expressed by using this system in this time. It may be necessary to compare the difference of characteristics of the gene sequence encoding amino acids between Chlamydia, E. coli and Brevibacillus, and so on, but this research is thought to be a help for chlamydial research in the future.
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| Free Research Field |
細菌学
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