The role of Interleukin-35 in experimental periodontitis model in Ebi3 knockout mice.

2017 Fiscal Year Final Research Report

• Project/Area Number: 16H07316
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Periodontology
• Research Institution: Aichi Gakuin University
• Principal Investigator: Goto Hisashi 愛知学院大学, 歯学部, 非常勤助教 (10783037)
• Research Collaborator: KIKUCHI Takeshi ; OKADA Kosuke
• Project Period (FY): 2016-08-26 – 2018-03-31
• Keywords: ノックアウトマウス / 実験的歯周炎

Outline of Final Research Achievements

The role of Interleukin (IL)-35 in periodontitis is not clear and the effect of IL-35 on the function of T helper 17 (Th17) cells is still incompletely understood. Therefore, we investigated the effects of IL-35, undertaken to compare experimental periodontitis induced by Porphyromonas gingivalis, clearly associated with the pathogenesis of periodontitis in Ebi3 knockout (KO) mice and wild-type (WT) mice. Computed tomography(CT) analysis and hematoxylin-and-eosin (H&E) and tartrate-resistant acid phosphatase (TRAP) staining were performed. Alveolar bone resorption was seen around the first and second mandibular molars in P.gingivalis-infected WT mice or in infected KO mice. Furthermore, infected Ebi3 KO mice alveolar bone resorption fenestration was higher than any other groups. Histological analysis of mandibles from Ebi3 KO and WT mice did not show clearly difference in the elements characteristic of periodontal tissues.

Free Research Field

歯周治療系歯学