2017 Fiscal Year Annual Research Report
Developing a novel high-throughput TCR-antigen pair identification method
| Project/Area Number |
16H07448
|
|---|
| Research Institution | Institute of Physical and Chemical Research |
|---|
Principal Investigator |
JIN JIANSHI 国立研究開発法人理化学研究所, 生命システム研究センター, 特別研究員 (80783018)
|
|---|
| Project Period (FY) |
2016-08-26 – 2018-03-31
|
| Keywords | T cell receptor / Antigen / High-throughput / Method |
|---|
| Outline of Annual Research Achievements |
In these months, I have cultured the T cells which express a specific T cell receptor, and the APCs (Antigen presenting cell) which present a specific antigen for the T cell. And also I have cultured another APC which presents a wrong paired antigen for that T cell as a negative control.
I have tried to identify the recognized T cell-APC pair in the mixture of T cells and APCs using a microscope. Using imaging, I have successfully found a pair of T cell interacted with its specific recognized APC. I also have tried to extract the T-cell-APC pairs from the solution.
For the high-throughput scanning, I have checked the scanning rate of the current microscope, it can scan more than 10^5 cells per 5 mins.
Until my cancellation of this grant, the other steps of my research plan are still ongoing.
|
| Research Progress Status |
29年度が最終年度であるため、記入しない。
|
| Strategy for Future Research Activity |
29年度が最終年度であるため、記入しない。
|
