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2017 Fiscal Year Annual Research Report

Evaluating the Influence of Viruses on the Deep Biosphere Carbon Cycle

Research Project

Project/Area Number 16H07489
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

PAN DONALD  国立研究開発法人海洋研究開発機構, 深海・地殻内生物圏研究分野, ポストドクトラル研究員 (90784851)

Project Period (FY) 2016-08-26 – 2018-03-31
Keywordsvirus / subsurface
Outline of Annual Research Achievements

1) While studying viruses from deep subseafloor sediment, I discovered that current virus techniques are not efficient at extracting viruses from the sediment. I developed a new method for separating viruses that can improve recovery of viruses from sediment by two orders of magnitude. This lowers the limit of detection so that I can detect viruses in sediment with extremely low biomass. This method was also successfully tested on viruses from iron mat samples. Data and experiments for the manuscript have been completed. The manuscript is currently in progress.
2) DNA sequencing of the subsurface coalbed virus community was completed. Bioinformatic analysis of the virus community is currently in progress. Assembly and annotation of the sequences is being performed.

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

  • Research Products

    (4 results)

All 2018 2017

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (2 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Exploring the Role of Relational Practices in Water Governance Using a Game-Based Approach2018

    • Author(s)
      Magnuszewski Piotr、Krolikowska Karolina、Koch Anna、Pajak Michal、Allen Craig、Chraibi Victoria、Giri Anil、Haak Danielle、Hart Noelle、Hellman Michelle、Pan Donald、Rossman Nathan、Sendzimir Jan、Sliwinski Maggi、Stefanska Joanna、Taillieu Tharsi、Weide Denise、Zlatar Ilonka
    • Journal Title

      Water

      Volume: 10 Pages: 346~346

    • DOI

      10.3390/w10030346

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Abundance and Distribution of Microbial Cells and Viruses in an Alluvial Aquifer2017

    • Author(s)
      Pan Donald、Nolan Jason、Williams Kenneth H.、Robbins Mark J.、Weber Karrie A.
    • Journal Title

      Frontiers in Microbiology

      Volume: 8 Pages: ~

    • DOI

      10.3389/fmicb.2017.01199

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Improved virus extraction and enumeration from deep subsurface sediment2017

    • Author(s)
      Donald Pan
    • Organizer
      International Society for Subsurface Microbiology 2017
    • Int'l Joint Research
  • [Presentation] Expanding the Frontiers of the Virosphere: Viruses in the Subsurface2017

    • Author(s)
      Donald Pan
    • Organizer
      Japan Geoscience Union - American Geophysical Union Join Meeting 2017
    • Int'l Joint Research / Invited

URL: 

Published: 2019-12-27  

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