Development of drug screening robot microscope by super resolution molecular dynamics analysis

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K00413
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Life / Health / Medical informatics
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: KOZUKA Jun 国立研究開発法人理化学研究所, 生命機能科学研究センター, 研究員 (10432501)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 1分子計測 / ロボット / 超解像

Outline of Final Research Achievements

In order to demonstrate a single-cell and single-molecule screening method, we established a single-cell and single-molecule measurement system related to the immune activity of mammals. These include TLR4-TIRAP signal transduction measurement system, S1P chemotaxis-molecular dynamics correlation measurement system, and IL6 stimulation-plasma cell differentiation in vitro experimental system. And then, a paper related to single molecule response of the TLR4-TIRAP signal transduction measurement system was accepted by JACS. In addition, I constructed two robot microscopes to develop single molecule measurement of single-cell. One is an upright and Inverted microscope that improves measurement efficiency by simultaneous measurement of two focal points, and the other is an incubation microscope that enables long time-lapse observation. In addition, the report of the developed microscopic system was accepted by Nat Commun.

Free Research Field

生物物理

Academic Significance and Societal Importance of the Research Achievements

人工知能支援顕微鏡法に基づき生細胞分析のために開発された自動単一分子イメージングシステムは、系統的な細胞シグナル伝達分析のための自動化単一分子イメージングが実行可能であり、単一分子スクリーニングに適用することができる。また、本研究課題において構築された哺乳動物の免疫活動に関係した１細胞・１分子計測系は、人間の疾病と直接関係が深いので、自己免疫疾患等の薬剤スクリーニングの実証系とても活用できる。したがって生物学的および薬理学的研究への広い貢献が期待できる。