2018 Fiscal Year Final Research Report
Innate immune responses in the silkworm
| Project/Area Number |
16K01018
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Science education
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| Research Institution | Nara University of Education |
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Principal Investigator |
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| Research Collaborator |
SUGIMURA yukio
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | 免疫 / カイコ / 高等学校 |
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| Outline of Final Research Achievements |
Insect immune system is divided into two major reaction types, cellular and humoral immune responses.
Actual protective responses were shown in the silkworm larvae, Bombyx mori, which had be anaesthetized on a frozen thermal gel and subsequently injected with various inocula. As a cellular response, phagocytic activity of hemocytes was observed 3 hr after injection of India ink, followed by accumulation of the ink carbon particles in hemocytes for successive 1 day.Chemical protection induced by humoral immune response was also examined, using larvae injected with heat-killed bacteria and bacterial lipo-polysaccharide (LPS). Antibacterial activities in hemolymph were detected constantly 6-24 hr after injection, which were assayed by the agar cup-plate method. Based on the present study, the topic of insect immune responses is thought to be accepted as a useful teaching material in high school.
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| Free Research Field |
生物教育
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| Academic Significance and Societal Importance of the Research Achievements |
カイコ卵や幼虫はインターネットを通じて購入でき、人工飼料でも飼育できるため、周年に亘り飼育できる。また、カイコ５齢幼虫は大きいので（体長７cm位），取り扱いが容易である．また， 1頭から400 μL体液が採取できるので、数名の生徒で共有して，実験を進めることができ，免疫実験の昆虫として都合がよい。本研究を基に，細胞性防御応答である食作用の観察と液性の化学的防御応答である抗菌タンパク質の検出を統合した実験を提案した．
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