2018 Fiscal Year Final Research Report
Mechanism of cell migration associated with dynamics of cell cytoskeleton and organelles by spatiotemporal control of cell shape
| Project/Area Number |
16K01350
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Chiba University |
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Principal Investigator |
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| Research Collaborator |
Nakanishi Jun
Nagayama Kazuaki
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | 細胞遊走 / 細胞内小器官局在 / 光応答性培養基板 / 細胞形状制御 |
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| Outline of Final Research Achievements |
In this study, the cell shape was micro-patterned into a triangle seen during migration using a photoresponsive culture substrate by UV light irradiation, and the localization of microtubules and centrosomes and the location of cell nuclei were observed by immunofluorescent staining. As a result, it was revealed that the centrosome localized in the lower part of the cell when the cell shape had polarity compared with the case where it was not patterned. After that, no change was observed in the localization of the centrosome after microtubule dissociation and reassembling, so it can be said that cell shape polarity, not microtubule distribution, affects the position of the centrosome in the cell height direction.
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| Free Research Field |
細胞遊走に関するバイオメカニクス
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| Academic Significance and Societal Importance of the Research Achievements |
細胞が基質上を這い回る細胞遊走の制御は，再生医療支援を始めとする次世代の医工学分野における基幹技術であり，その確立のためには遊走メカニズムの解明は必要不可欠である．特に，細胞形状の非対称性，すなわち細胞極性の形成や，核と微小管形成中心を結ぶ軸が細胞遊走と関連することは知られていたものの，それらを理論立てて裏付ける研究は皆無であり，細胞遊走メカニズムの全容解明には程遠い状況であった．そのため，これからの医工学分野において本研究成果の学術的意義および社会的意義は大きい．
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