Development of soft nanoparticle composite materials based on decellularized tissues and its application to treatment of myocardial infarction

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K01387
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: HASHIMOTO YOSHIHIDE 東京医科歯科大学, 生体材料工学研究所, 助教 (40638384)
• Co-Investigator(Kenkyū-buntansha): 根岸 淳 信州大学, 繊維学部, 助教(特定雇用) (60722634)
• Research Collaborator: Funamoto Seiichi ; Yamashita Akitatsu ; Akiyoshi Kazunari
• Project Period (FY): 2016-10-21 – 2019-03-31
• Keywords: ナノゲル / 脱細胞化生体組織 / ECMハイドロゲル

Outline of Final Research Achievements

we have developed decellularized porcine myocardium, bladder matrix (UBM), and small intestinal submucosa (SIS) by surfactant method and high-hydrostatic pressure (HHP) method. The tissue structure of decellularized tissue prepared by HHP method was maintained compared with surfactant method.
We have investigated the efficacy of nanogel-crosslinked gel or nanogel-crosslinked gel/UBM-ECM gel composite material using rat myocardial infarction model. As a result, it revealed that nanogel-crosslinked gel/UBM-ECM gel suppressed the myocardial thinning and fibrosis.

Free Research Field

バイオマテリアル

Academic Significance and Societal Importance of the Research Achievements

本研究で提案する脱細胞化組織-ナノゲル複合材料は、脱細胞化組織に存在する増殖因子などの薬物をナノゲルが取り込み、徐放するDDS機能と細胞の三次元足場として働く機能を有する。この材料は、心筋梗塞で心機能が低下した多くの患者のQOLの改善に役立つ可能性が期待できる。また、様々な組織の再生に応用可能であるばかりか、種々の幹細胞の機能制御に新たなツールを与え、iPS 細胞などを用いた再生医療の発展に貢献することが期待される。