2018 Fiscal Year Research-status Report
Oxidative stress in skeletal muscle exercise and injury
| Project/Area Number |
16K01736
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2021-03-31
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| Keywords | redox / exercise / injury / imaging / roGFP |
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| Outline of Annual Research Achievements |
Physical exercise enhances fitness and overall health and wellness. Bouts of aerobic and anaerobic exercise induce an acute state of oxidative stress caused by an increase in reactive oxygen species (ROS), but there is little agreement which processes generate ROS and where, if they are important and how they are controlled. We produced transgenic mice expressing redox-sensitive GFP in skeletal muscle, and set up imaging systems to record muscle redox state in live anesthetized animals. Determining the conditions necessary for induction of oxidative stress during skeletal muscle activation in mice in vivo, preliminary experiments indicated that some amount of muscle fiber injury is necessary to induce oxidative stress during skeletal muscle activation. Simple electrical stimulation of muscle did not induce oxidation of the skeletal muscle glutathione pool. Injured muscle fibers became oxidized, but this oxidation did not spread to adjacent fibers in the absence of muscle activation.
Experiments to publish our finding that muscle activation without injury does not result in oxidative stress were finished, but experiments using controlled injuries did not yield satisfactory results, and alternative strategies are under consideration.
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| Current Status of Research Progress |
Current Status of Research Progress
4: Progress in research has been delayed.
Reason
After a change in department head and research orientation of the department, the principal investigator (PI) relocated to a different department more suitable to continue this research.
At the same time, the 5th child of the PI was born and the PI decided to take parental leave from December 2018 to December 2019.
After consulting with the research promotion division of the university, the research project was prolonged rather than stopped for the duration of parental leave, in order to permit spending during this parental leave period.
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| Strategy for Future Research Activity |
Research will be delayed until the principal investigator (PI) returns from parental leave, most probably in January 2020.
From January 2020, time will be spent on setting up in the new research environment and preparing for publication.
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| Causes of Carryover |
Delay due to parental leave. Remaining amount is planned to be used for publication costs.
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