2020 Fiscal Year Research-status Report
Oxidative stress in skeletal muscle exercise and injury
| Project/Area Number |
16K01736
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2022-03-31
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| Keywords | redox / exercise / injury / imaging / roGFP / DAMPs / 運動 / 酸化ストレス |
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| Outline of Annual Research Achievements |
Physical exercise enhances fitness and overall health and wellness. Bouts of aerobic and anaerobic exercise induce an acute state of oxidative stress caused by an increase in reactive oxygen species (ROS), but there is little agreement which processes generate ROS and where, if they are important and how they are controlled. We produced transgenic mice expressing redox-sensitive GFP in skeletal muscle, and set up imaging systems to record muscle redox state in live anesthetized animals. Determining the conditions necessary for induction of oxidative stress during skeletal muscle activation in mice in vivo, preliminary experiments indicated that some amount of muscle fiber injury is necessary to induce oxidative stress during skeletal muscle activation. Simple electrical stimulation of muscle did not induce oxidation of the skeletal muscle
glutathione pool. Injured muscle fibers became oxidized, but this oxidation did not spread to adjacent fibers in the absence of muscle activation.
Experiments to publish our finding that muscle activation without injury does not result in oxidative stress were finished, but experiments using controlled injuries did not yield satisfactory results. Alternative strategies were considered, but did not reach publishable results.
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| Current Status of Research Progress |
Current Status of Research Progress
4: Progress in research has been delayed.
Reason
理由
The principal investigator (PI) prolonged his parental leave (which started from December 2018) again until December 2020.
As a consequence, the project was stopped for the 2 years duration of parental leave and will resume once parental leave ends.
研究代表者が 5人目の子供の誕生で 2018年12月より取得していた育児休業を 2020年12月まで再延長した。
育児休業の 開始年月日及び 終了年月日： 平成３ ０ 年１ ２ 月２ ７ 日 ～ 令和２ 年１２ 月２ ６ 日
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| Strategy for Future Research Activity |
The PI was on parental leave during most of the reporting period.
Research continued after the principal investigator (PI) returned from parental leave.
育児休業終了後に研究活動を再開した。
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| Causes of Carryover |
育児休業によって研究活動が中断したので次年度使用額が生じた。
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