2016 Fiscal Year Research-status Report
Bio-friendly nanosheets loaded with laser-generated nano-containers that release metal ions to accelerate wound healing
| Project/Area Number |
16K04904
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| Research Institution | Tokai University |
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Principal Investigator |
クリニッチ セルゲイ 東海大学, 創造科学技術研究機構, 准教授 (00623092)
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| Co-Investigator(Kenkyū-buntansha) |
岡村 陽介 東海大学, 工学部, 准教授 (40365408)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | Laser ablation / Nanoparticles / Nanocontainers / Polymer nanosheets / Ion release / Burn wound healing |
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| Outline of Annual Research Achievements |
During the 1st year, we worked on: (1) Preparing various Zn-containing nanoparticles (metallic Zn, ZnO, ZnCl2) via laser ablation in liquids (water, ethanol, chloroform). (2) Studying their properties.(3) Preparing poly-L-lactic acid (PLLA) nanosheets embedded with such nanoparticles, and characterizing prepared nanosheets. (4) Evaluating how Zn ions are released from nanosheets into liquid medium over time. The dynamics of Zn ion release from prepared nanosheets was evaluated upon placing the nanosheets into buffer solution with pH 7.4 and at temperature of 37 C. Interestingly, all nanosheets demonstrated very similar Zn ion release dynamics, and similar Zn ion concentrations were achieved in the buffer (irrespective of Zn containing nanoparticles used), when similar amounts of nanoparticles were embedded. Importantly, maximal Zn ion concentrations were achieved within one day after the nanosheets were placed into the buffer, which looks attractive from biomedical point of view. (5) In addition, we tested certain ZnO nanoparticles prepared in water as gas sensing element and obtained promising results.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
After one year, the project is proceeding smoothly and mainly according to initial plans. (1) As planned, during year one, all basic work and methodologies were developed based on Zn -containing nanoparticles (metallic zinc, zinc chloride, zinc oxide nanoparticles). (2) Methodologies for nanoparticle and nanosheet preparation, as well as for Zn ion release evaluation were well established. (3) One journal paper was published, and several conference presentations were made. (4) Using laser-generated ZnO nanoparticles, we obtained promising gas sensing results. (5) Experiments in vivo are now to be conducted to test how Zn-ion incorporated nanosheets influence burn wound healing in comparison with un-doped nanosheets. (6) We are now about to start work on nanoparticles based on other elements (e.g. Mg, Fe).
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| Strategy for Future Research Activity |
During the current year, we plan to continue the research related to the project as: (1) Preparation of Mg and Fe containing nanoparticles via laser ablation in different liquids (water, ethanol, chloroform). (2) Characterization of the prepared nanomaterials and incorporating them into PLLA nanosheets. (3) Evaluating how Mg/Fe ions are released from nanosheets into liquid medium over at pH 7.4 and at temperature of 37 C. (4) Testing the effect of prepared nanosheets (incorporated with various nanoparticles) in vivo (on animals).
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| Causes of Carryover |
Since during year 1 somewhat less than planned money was spent on equipment, this year we plan to purchase some equipment. A USB spectrometer for photoluminescence measurements to evaluate properties of produced nanomaterials is one of our targets this year. Another possible tool, depending on funds and their availability, is a light source with a wide spectral range which can be used as an excitation source (e.g. Xe lamp). The other expenses planned for the year are: (i) consumables (of which a considerable part will probably be spent on in vivo tests on animals), (ii) conferences (one domestic and one overseas trips are planned), and (iii) analyses.
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| Expenditure Plan for Carryover Budget |
USB spectrometer: 430,000 yen. Light source (and filters), Xe lamp or similar: 200,000 yen. Animals for in vivo tests: 70,000 yen. Other consumables: 200,000 yen.
Conferences, including tickets and registration: 400,000 yen.
Sample analyses : 200,000 yen.
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Research Products
(10 results)
