2017 Fiscal Year Research-status Report
Bio-friendly nanosheets loaded with laser-generated nano-containers that release metal ions to accelerate wound healing
| Project/Area Number |
16K04904
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| Research Institution | Tokai University |
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Principal Investigator |
クリニッチ セルゲイ 東海大学, 創造科学技術研究機構, 教授 (00623092)
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| Co-Investigator(Kenkyū-buntansha) |
岡村 陽介 東海大学, 工学部, 准教授 (40365408)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | laser ablation in liquid / nanoparticles / polymer nanosheets / biomedical material / burn wound |
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| Outline of Annual Research Achievements |
During year two, we worked on:
(1) Preparing various Mg-containing nanoparticles (metallic Mg, MgO, MgCl2) via laser ablation in liquid phase (water, ethanol, chloroform). (2) Preparing various Zn-containing nanoparticles (metallic Zn, ZnO, ZnCl2. (3) Preparing poly-L-lactic acid (PLLA) nanosheets embedded with such nanoparticles (both Zn- and Mg-containing types), and characterizing prepared nanosheets. (4) Evaluating how Mg ions are released from nanosheets into physiological solution over time. The dynamics of Mg ion release from prepared nanosheets was evaluated upon placing the nanosheets into buffer solution with pH 7.4 and at temperature of 37 C. The tested nanosheets demonstrated very fast Mg ion release dynamics: maximum concentrations were achieved within just a few hours. This was much faster than ion release observed from Zn-containing PLLA nanosheets studied before. (5) In addition, we tested certain ZnO and SnO2 based nanoparticles, as well as their hybrids, prepared in water as gas sensing elements or photocatalysts for degradation of organic compounds (gases and liquids) and obtained promising results.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
After one year, the project proceeded with some delay. (1) As planned, during year two, we switch our attention to Mg-containing nanoparticles prepared via laser ablation in liquid phase (metallic, oxide, chloride). (2) Such nanoparticles were prepared and incorporated into polymer nanosheets, after which the release of Mg ions from such nanosheets into physiological solution was evaluated. (3) Three journal papers were published, and several conference presentations were made. (4) Using laser-generated SnOx, ZnO and/or SnOx-ZnO nanoparticles, we obtained promising gas sensing results. (5) However, in vivo experiments (with Zn-ion and Mg-ion incorporated nanosheets and their influence on burn wound healing) were delayed. This is explained by increasingly stricter ethical requirements imposed onto in vivo tests on animals, and thus the necessity to find new test protocols that suit our demands. (6) At the same time, we started experiments on antibacterial performance of Zn- and Mg-containing nanosheets, as antibacterial behavior during wound healing seems very promising.
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| Strategy for Future Research Activity |
During year three, we plan to continue the research related to the project as follows:
(1) Preparation of Cu-, Mg- and Fe-containing nanoparticles via laser ablation in different liquids (chloroform, ethanol, water). (2) Characterization of the prepared nanoparticles and their incorporation into PLLA nanosheets. (3) Evaluation of how Cu/Mg/Fe ions are released from nanosheets when in contact with liquid medium with pH 7.4 and temperature 37 C. (4) Testing how PLLA nanosheets incorporated with Zn-, Cu-, Mg-, or Fe-containing nanoparticles affect burn wound healing in vivo (on animals). (5) Preparing PLLA nanosheets incorporated with 2 (and more) nanoparticle types and testing their effect on wound healing.(6) Adding Ag-containing nanoparticles into PLLA nanosheets, as additional nanoparticle source aiming at providing antibacterial effect, and testing the effect of Ag-containing nanoparticles in combination with other nanoparticles.
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| Causes of Carryover |
Since during years 1-2 less than initially planned money was spent on equipment, this year we plan to purchase somewhat more. A spin-coater for thin-film preparation is one of our main targets this year. We also think of a light source with a wide spectral range which can be used as an excitation source (e.g. Xe lamp). The other expenses planned for the year are: (i) consumables (of which a considerable part will probably be spent on in vivo tests on animals), (ii) conferences (1-2 domestic and one overseas trips are planned), and (iii) analyses.
Spin-coater: 600,000 yen, wide-range light source (Xe lamp or analog), including filters: 300,000 yen. Animals for in vivo tests: 100,000 yen. Other consumables: 300,000 yen. Conferences, including tickets and registration: 450,000 yen.
Sample analyses: 200,000 yen.
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