Development of high-precision sugar sensing systems based on differential signal detection by photochromism

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K05810
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: University of Toyama
• Principal Investigator: TOHDA Koji 富山大学, 大学院理工学研究部(工学), 教授 (60212065)
• Research Collaborator: KANNO Akira
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 血糖値センサ / 差分検出法 / フォトクロミズム / 光リセット / 光応答性ゲル / オプティカルpHセンサ / オプティカルグルコースセンサ

Outline of Final Research Achievements

In order to improve the precision of wearable optical sensors that can monitor blood glucose levels in subcutaneous interstitial fluid in a minimally invasive manner, we have developed a high-precision sugar sensing system based on differential detection of sensor responses by photochromism. In spiropyran-copolymerized photochromic films, spiropyran exists in pH sensitive merocyanine form, but it is isomerized and insensitive to pH when irradiated with visible light. Based on this photochromic film and glucose oxidase, we constructed a glucose sensor with pH change as an indicator of response. This sensor is capable of differentially detecting glucose with high precision while completely canceling out optical background fluctuations.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

フォトクロミズムをオプティカルセンサシグナルのon/offに用い，そのシグナル差分を検出することによってバックグラウンドシグナルの影響を除去するという試みは，これまで一切なされていない。本法は同一のセンサからセンシングシグナルと参照シグナルの両方を取得するものであり，バックグラウンドの変動の影響を受けない精度の高い測定が可能で，皮下滞在型光学式血糖値センサの実用化に大きく寄与するものと期待できる。