Simple and high-performance diagnostics chip using paper, film and tape

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K05833
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Fuchiwaki Yusuke 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (80468884)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: チップ / キット / イムノクロマト / 紙

Outline of Final Research Achievements

Although paper-based microfluidic chip devices are an ideal platform for point-of-care diagnostics, it is difficult to achieve microfluidic control required for sensitive analyses such as ELISA on a paper substrate. In this study, we developed a novel lateral-flow test chip that can perform operations like a pump, such as flowing, stopping, and replacing a solution, just by adding the solution onto an inlet. For sensitive and accurate detection, the transparency and flatness of the substrate is crucial for precise analysis of weak light generated by a specific reaction. The signal intensity of the novel chip was higher than that of a chip made of nitrocellulose membrane, and the variation was smaller. The limit of detection of the chip was 0.1 ng/ml, whereas that of the nitrocellulose membrane was 100 ng/ml. In addition, we succeeded in developing a compact prototype equipment by combining these technologies.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

本研究では、安価な紙・フィルム・テープから構成される新しい分析チップを開発し、液の滴下のみの操作で、発色と酵素免疫測定法による高感度検知に成功した。また、臨床分野の簡易検査におけるゴールドスタンダードのイムノクロマト法に比べて、100倍以上高感度に測定できることを実証した。途上国や緊急時の医療検査では、費用、耐久性、使い易さが大きな障害となっていたが、液の滴下だけの操作であれば専門知識のない人でも手軽に使えることから本法は実用化に向けて卓越したメリットがある。また多くの市販のキット組みわせることにより高い選択性と幅広い検査への展開も可能であるため、多様な学術分野への水平展開が強く期待される。