Development of a platform for bio-production of useful compounds using S-adenosylmethionine-dependent enzymes

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K06864
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Hokkaido University
• Principal Investigator: Satoh Yasuharu 北海道大学, 工学研究院, 助教 (30360928)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: S-アデノシルメチオニン / メチル基転移酵素 / 補酵素 / 葉酸 / 代謝工学

Outline of Final Research Achievements

Microbial production of various useful compounds has been reported using synthetic biology and metabolic engineering methods. To further expand the products, application of S-adenosylmethionine-dependent enzymes are important. Therefore, this study aimed to develop a platform for utilizing these enzymes. As the results, it was confirmed that phenylethanolamine-N-methyltransferase was useful for the verification. To avoid inhibition by the by-product S-adenosylhomocysteine, the degradation pathway of Escherichia coli could be used.

Free Research Field

生物化学工学

Academic Significance and Societal Importance of the Research Achievements

S-アデノシルメチオニン依存メチル基転移酵素を用いた有用物質生産はすでに報告されているが (鎮痛剤モルヒネ等)、その生産量は数十μMと非常に低く、内在性SAMで十分賄えるレベルであった。しかし生産量が増えた場合、SAM供給不足だけでなく反応によって生じる副産物S-アデノシルホモシステインによる反応阻害も無視できない。したがって、供給系と分解系を強化したプラットフォームは今後のバイオプロセス開発において解決すべき重要な課題といえる。