Tfcp2l1 confers robust self-renewal in embryonic stem cell

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K07104
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Kanazawa Medical University
• Principal Investigator: OHTSUKA Satoshi 金沢医科大学, 総合医学研究所, 准教授 (40360515)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: Tfcp2l1 / ES細胞 / LIFシグナル / マウス系統差

Outline of Final Research Achievements

Mouse embryonic stem cells (mESCs) have been established in conventional serum culture. mESCs derived from 129 mouse strain are stably self-renewing in serum culture. In contrast, mESCs from non-obese diabetic (NOD) mouse strain is not yet maintained in serum culture. In this project, we tried to explore why ESCs from 129 strain were stably established and continued to self-renew in serum culture. We identified transcription factor Tfcp21 which was specifically retained in 129-ESCs. Maintaining the expression of Tfcp21l in 2i-established NOD-ESCs allowed self-renewal in an inhibitor-free serum condition. Tfcp2l1 enhanced LIF-Stat3 activity in NOD-ESCs at a similar level to that in 129-ESCs. These results indicate that Tfcp2l1 supports 129-ESCs self-renewal in serum through enhancing LIF-Stat3.

Free Research Field

幹細胞生物学

Academic Significance and Societal Importance of the Research Achievements

本研究で得られた成果をもとに、ナイーブ型幹細胞の樹立と維持が、ヒトを含む哺乳類からも可能となり、再生医学へ大きく貢献できると考えている。