Generation of homoplasmic mtDNA modified cell library

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K07207
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genome biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Wada Ken-Ichi 国立研究開発法人理化学研究所, 開拓研究本部, 協力研究員 (20525919)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: mtDNA / マイクロデバイス

Outline of Final Research Achievements

Single mitochondrion transfer is a possible approach for homoplasmic mtDNA modification. This study aimed to develop a novel method for single mitochondrion transfer and following mtDNA cloning to achieve homoplasmic mtDNA modification. In this study, I succeeded in performing single mitochondrion transfer between live single cells by achieving cell fusion through a 10 μm-length microtunnel using a microfluidic device, and in harvest of mitochondria transferred mtDNA-less cell from the microfluidic device. This provides an important basis for development of homoplasmic mtDNA modification method.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

実用的なmtゲノム改変技術は開発されていないため、mtゲノムの機能解析の実施には大きな制約がある。ホモプラズミックなmtゲノム改変の実現と、それに引きつづいてmtゲノム改変細胞ライブラリーを構築することによって、これまで制約の多かったmtゲノム解析を強力に推進する研究プラットフォームの創出が期待される。