2017 Fiscal Year Research-status Report
An innovative approach of using wild relatives genomics for the climate-resilient crop development and conservation
| Project/Area Number |
16K07234
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| Research Institution | Kyushu University |
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Principal Investigator |
Firouzeh Javadi 九州大学, 持続可能な社会のための決断科学センター, 助教 (70640910)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | Wild species / Habitat / Geographical group / Cicer |
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| Outline of Annual Research Achievements |
The distantly related wild cousins (DWRC) of cultivated plants can be seen as a “huge natural mutagenesis resource” with novel gene variants that can increase productivity, quality, and adaptability. Nevertheless, distantly related wild species are given less priority and genomic tools reveal the secrets of the genetic potential of wild relatives to respond to a range of environments. We address this need in chickpea, an important legume, by harnessing the diversity of DWRC. In the second fiscal year, we analyzed morphological characters of the germinated seeds in greenhouse. The seeds of some species didn’t germinate. We therefore used morphological characters from the collected herbarium specimens from different herbaria. Phylogenetically related species from a geographical and habitat showed characteristic feature of the seed coat texture, leaves traits, and growth habit. Outcomes of the results will be presented in International conferences.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Morphological evaluation and genetic analyses are progressing smoothly. The seeds of some species didn’t germinate. The field survey in Iran and Turkey postponed due the official process. However, National Plant Genebank of Iran provided recent field survey information and seed samples for the analyses. We therefore expect the seed samples and information will be sufficient for genomic and phylogenetic analyses using double digest restriction-site associated DNA (ddRADseq).
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| Strategy for Future Research Activity |
In the next fiscal year, the morphological evaluation will be continued. We will also include morphological data of the herbarium specimens from different herbaria. ddRAD-seq and genotyping will be performed to characterize the adaptive diversity of of distantly related wild of chickpea and to understand genetic composition of rare and endangered species.
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| Causes of Carryover |
The ddRAD-seq generates a data set consisting of several thousand genetic markers distributed throughout coding and non-coding parts of the genome. These dataset as well as morphological and ecological data will allow for a fine-scale assessment of population structure, identify the relative importance of microevolutionary processes, and genomic composition of rare and threatened species. The major expenses will be required for genomic analysis. The travel expenses for participating in the conference are needed.
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Research Products
(2 results)
