Analysis of nitrogen dynamics in rice plants.

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K07578
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Crop production science
• Research Institution: Ishikawa Prefectural University
• Principal Investigator: Tsukaguchi Tadashi 石川県立大学, 生物資源環境学部, 准教授 (40345492)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: イネ / 窒素利用効率 / タンパク質含量 / 子実窒素集積性

Outline of Final Research Achievements

We hypothesize that nitrogen allocation to rice grains is mostly determined by the grain-protein-accumulation ability which is genetically determined and is not affected by environments and aim to clarify the grain-protein-accumulation ability genetically and physio-ecologically. We developed a quantitative measure for the evaluation of grain-protein-accumulation ability, compared it among rice cultivars and selected two cultivars, one with high and one with low grain-protein-accumulation ability. Using these cultivars as parents we developed F2 and F3 populations. QTL (quantitative trait loci) analysis with these populations revealed that several QTLs contributed grain-protein-accumulation ability. Among these QTLs, two QTLs were detected in both generations and both nitrogen conditions. We now are developing near isogenic lines with the introgression of chromosome segments of the other parent’s genotype at these two QTL regions to validate the effect of these QTLs.

Free Research Field

作物学

Academic Significance and Societal Importance of the Research Achievements

本研究により、子実窒素集積性は他の遺伝形質や環境要因ともに、子実のタンパク質含量、登熟期のイネ体窒素動態に寄与することが分かった。イネの子実タンパク質含量は栄養的および食味的な側面から重要な形質である。また、持続的な農業生産や環境負荷を最低限にしながら収量性を高めることが重要でなり、イネ体の窒素利用効率の高い品種の育成が求められるが、そのためにはイネ体窒素動態の解明が不可欠でかる。本研究の成果はそれらを目的とした品種育成に寄与することが期待される。