Clarification of changes of microbiota, which sustain intensities of indigo-reduction in indigo fermentation

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K07684
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Yumoto Isao 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究部門付 (30358303)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: インジゴ還元 / 発酵の前処理 / 藍染め / 微生物叢 / Alkalibacterium / Amphibacillus

Outline of Final Research Achievements

Occurrence of indigo-reduction within 10 days after the preparation and the maintenance of the reduced state longer are important in indigo fermentation. Carrying out appropriate treatments according to the grasping the fermentation situation are also important for its lasting for long period. A purpose of this study is understanding followings: changes of microbiota in the initiation of reduction of indigo, the influence of substrate addition for the microbiota and relationship between staining intensity and the microbiota. It is considered that the obtained results will realize long-lasting indigo-fermentation. The examined results indicated that appropriate pretreatment procedure is important to adjust the desirable microbiota. In addition, the influence that substrate addition on the microbiota and dyeing intensity related microbiota were clarified.

Free Research Field

微生物生理生態学

Academic Significance and Societal Importance of the Research Achievements

発酵の初期の高温、高アルカリ性の処理が、発酵に必要な微生物を選抜し、存在が好ましくない微生物を排除していることを明確に示すデーターが得られた。これは多くの発酵の前処理の意味をハッキリしたデーターとして示した非常に貴重なデーターと考えられる。また、短期間で還元能力が低下した系と長期間還元状態を維持した系を比較することによって発酵液の微生物叢のあるべき姿を通じて、藍発酵液を長期間維持するためにどの様なメンテナンスを施すべきかを明らかにした。このことは、他の自然発酵系の維持にも応用出来るものと考えられる。