2018 Fiscal Year Final Research Report
Protein engineering studies on stable cytochrome c' aiming at enhancing NO binding
| Project/Area Number |
16K07692
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
河合 幸一郎 広島大学, 生物圏科学研究科, 教授 (30195028)
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| Project Period (FY) |
2016-10-21 – 2019-03-31
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| Keywords | NOセンサー / NO結合 / 蛋白質 / シトクロムｃ' |
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| Outline of Final Research Achievements |
This study was aiming at enhancing NO binding ability of thermally stable cytochrome c', PHCP, using acidophilic midge haemoglobin as a model. Through this study, we succeeded in estimating NO binding affinity by measuring absorption spectral changes upon NO binding with PHCP, and in enhancing NO binding ability of PHCP by introducing mutations into PHCP. The resulting PHCP variants will be promising materials to be used as NO sensor proteins.
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| Free Research Field |
蛋白質科学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は，代表者が専門とする蛋白質科学分野にユスリカの形態分類学を導入した点で，異分野を融合する学術的に独創的な試みであった。本研究の成果として得られた耐熱性を保持しNO結合能が向上した蛋白質（PHCP変異体）は，血管弛緩などの生理作用を示すNOを感度よく測定できるNOセンサー蛋白質として利用できる可能性があり，ひいてはNOをマーカーとして身体ストレスを簡便に検出できる意義がある。
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