Study of pancreatic islet formation during development by long-term time-lapse imaging

2019 Fiscal Year Final Research Report

• Project/Area Number: 16K08090
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Kansai Medical University
• Principal Investigator: YOSHIDA Naoko 関西医科大学, 医学部, 講師 (40392170)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Keywords: ノックインマウス / 膵臓 / 膵島 / 内分泌細胞 / 多色細胞系譜追跡実験 / Sox9

Outline of Final Research Achievements

To investigate pancreatic islet formation during mouse development, we first attempted to produce an unique knock-in mouse which pancreatic endocrine progenitors would be labeled specifically. In spite of the achievement in obtaining several homologouslly recombined ES cell clones, we failed to establish an knock-in mouse. According to this, we carried out long-term 3D time-lapse imaging using a different knock-in mouse which pancreatic progenitors would be labeled specifically. Based on the multi-color lineage tracing of ex vivo cultured pancreatic buds, we found that one single islet is derived from more than three different pancreatic progenitor cells.

Free Research Field

発生生物学

Academic Significance and Societal Importance of the Research Achievements

樹立した相同組換え体ES細胞は、膵内分泌前駆細胞を特異的に標識できるノックインマウスの新規作製のみならず、分化誘導実験に応用利用することが可能であり、有用な実験材料を得たと言える。また、複数の膵前駆細胞を起源とした内分泌細胞の集団化によって膵島が構築されることを示す直接的証拠が得られた。膵島形成機構に関するこのような知見を集積することは、将来的にiPS細胞を用いた移植再生医療への貢献に繋がるものと期待される。