2017 Fiscal Year Research-status Report
Elucidation of molecular mechanisms for transduction of jasmonate signal to fertility in rice
| Project/Area Number |
16K08143
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| Research Institution | Okayama University |
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Principal Investigator |
Ivan Galis 岡山大学, 資源植物科学研究所, 教授 (90360502)
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| Project Period (FY) |
2016-10-21 – 2019-03-31
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| Keywords | rice / flower / development / hormone / fertility / plant defense / jasmonic acid |
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| Outline of Annual Research Achievements |
After investigation of Osjar1 mutant lacking JA-Ile biosynthetic capacity, we further replicated the FY28 data using hebiba mutant plants that are impaired in an earlier step of jasmonate biosynthesis. We successfully confirmed jasmonate profiles in flowers, as well as FY28 data on MADS box expression in an independent experimental system. In FY28/29, we also identified first regulator, a NAC type transcription factor, which was expressed in flowers and down-regulated in Osjar1 flowers in jasmonate-dependent manner. We constructed transgenic plants in FY29 and the phenotype will be examined in detail in FY30. Next, we conducted microarray experiment as planned using Osjar1 and wild type flowers with flower material that expressed maximal jasmonate levels in wild type (as determined in FY28) to identify genes regulated by this hormone using their differential expression in the Osjar1 mutant. Two independent lines on Osjar1 were used and we successfully obtained several candidate regulators consistently downregulated in jasmonate-dependent manner. In particular, a novel Oryza sativa MYB/MADS genes was almost 100x downregulated in Osjar1 flowers. We focus on this gene in FY30 and construct transgenic plants silenced in the expression of MYB/MADS domain gene. Flower phenotype will be examined in FY30. In additional experiments, we focused on metabolic profiles in flowers as potential targets of jasmonates, which normally function as defense regulators in leaves and other tissues. We extensively studied signaling mechanisms in rice defense.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
In the initial year, the progress was slightly behind due to late date of approval of this grant (10/21/2016), causing a 6 month delay of start. However, we were able to catch up with the original plan in all main areas, conduct all necessary screening procedures, so we are now in progress of construction of transgenic plants to functionally characterize novel jasmonate-regulated genes in rice flowers. We expect to reach our final goal, which is explanation of molecular function of jasmonates in fertility of rice by the end of the project. We also gained much progress in understanding of rice defense mechanisms, which is essential for successful interpretation of overall results obtained in this project.
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| Strategy for Future Research Activity |
We focus on transformation procedures and generation of plants silenced in the expression of candidate genes found in FY29. Flowers will be characterized at morphological and metabolic levels to reveal the function of newly identified jasmonate-dependent genes in rice flowers. The transfer budget will be used to employ additional technical staff necessary for achieving good progress in FY30.
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| Causes of Carryover |
We have not employed a part time worker as expected in the whole FY29, which resulted in surplus of budget and its transfer to FY30. Also due to 6 month delayed start in FY28, some of the funds accumulated for final year that will enable more aggressive work in the last stage of the project.
The budget will be used to employ additional technical staff necessary for achieving successful progress in FY30. Also, funds will be used for attendance of the international conference on chemical ecology (Hungary), and presentation of our latest results (abstracts applied for August 13-17, 2018).
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[Journal Article] Integration of danger peptide signals with HAMP signaling amplifies the anti-herbivore defense responses in rice2018
Author(s)
Shinya T, Yasuda S, Hyodo K, Tani R, Hojo Y, Fujiwara Y, Hiruma K, Ishizaki T, Fujita Y, Saijo Y, Galis I
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Journal Title
The Plant Journal
Volume: In press
Pages: In press
DOI
Peer Reviewed
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[Presentation] Omics everywhere: How about in chemical ecology?2017
Author(s)
Ivan Galis, Tomonori Shinya, Kabir Md. Alamgir, Islam Sobhy, Kimiaki Tanabe, Atsushi Miyake, Yuko Hojo, Cyprian Osinde, Wataru Sakamoto, Ryo Nakabayashi, Kazuki Saito
Organizer
33rd Annual meeting of the International Society for Chemical Ecology and the 9th meeting of the APACE
Int'l Joint Research / Invited
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