Development of a method for the analysis of protein O-GlcNAcylation using two-step click chemistry

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K08219
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology
• Principal Investigator: Tsumoto Hiroki 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (00409385)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: O-GlcNAc / プロテオミクス / 質量分析

Outline of Final Research Achievements

In this study, a method for the enrichment of O-GlcNAcylated peptides using two-step click chemistry was developed. Analysis of O-GlcNAcylated proteins was performed using extracts from rat kidney tissue. A total of 34 O-GlcNAcylated peptides from 28 proteins were identified. Of these, 2 proteins were increased and 7 proteins were decreased in diabetic rat.

Free Research Field

プロテオミクス

Academic Significance and Societal Importance of the Research Achievements

タンパク質のO-GlcNAc化は血糖値によって変化することから、糖尿病において重要な修飾である。本研究課題により、糖尿病モデル動物の腎臓で増加あるいは減少するO-GlcNAc化タンパク質の同定に成功した。今後、これらの生物学的な意義を明らかにすることで、糖尿病性腎症の病因・病態の解明に繋がる可能性がある。