Regulation of cell division by tyrosine phosphorylation signaling

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K08253
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Kyoto Pharmaceutical University
• Principal Investigator: Nakayama Yuji 京都薬科大学, 薬学部, 教授 (10280918)
• Co-Investigator(Kenkyū-buntansha): 齊藤 洋平 京都薬科大学, 薬学部, 助教 (90411032) ; 久家 貴寿 京都薬科大学, 薬学部, 助教 (20551857)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 細胞分裂 / チロシンリン酸化 / v-Src / 細胞周期

Outline of Final Research Achievements

To elucidate the mechanisms underlying tyrosine phosphorylation-mediated regulation of cell division, we performed proteomic analysis using cell cycle-synchronized cells, screening of inhibitors affecting progression of cell division, and analysis of effect of v-Src on cell division, as a model of abnormally activated tyrosine phosphorylation signal. We found possible tyrosine phosphorylation of intermediate filament in cytokinesis, and delay in M-phase progression by inhibitors against receptor-type tyrosine kinases. Furthermore, v-Src causes premature mitotic exit and restart of cell cycle of cells having increased DNA content, leading to abnormal cell division in the following cell cycle.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

細胞分裂制御関連タンパク質の機能異常は染色体不安定性を介して細胞のがん化に関与するため，新規制御機構の解明は，新たな細胞がん化機構の発見につながる。また，細胞分裂制御に関わるタンパク質は，抗がん剤を開発するための標的分子となる可能性がある。