2018 Fiscal Year Final Research Report
Cellular dynamics and molecular mechanism on lymphatic network formation in artificial three-dimensional human tissue
| Project/Area Number |
16K08459
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Research Collaborator |
WATANABE SEIJI
NARITA HIROKAZU
OKANO DAISUKE
MATSUSAKI MICHIIYA
AKASHI MITSURU
YOSHIMATSU YASUHIRO
WATABE TETSUROU
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | 三次元ヒト生体組織 / リンパ管ネットワーク / セルダイナミズム |
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| Outline of Final Research Achievements |
The present study established in vitro 3D-human tissue model involving lymphatic vascular network by our developed cell-accumulation method, and investigated morphogenesis and molecular mechanism in lymphatic network formation. The lymphatic endothelial cells showed cellular dynamics featuring cell extensions, connections and separations in the 3D-human tissue, and eventually constructed three-dimensional network. The cells further displayed a specific expression of various functional molecules in phase with their dynamics, and the intimate control of the lymphatic morphogenesis by the molecular expressions in accordance with the time line were demonstrated.
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| Free Research Field |
医歯薬学
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| Academic Significance and Societal Importance of the Research Achievements |
独自の技術によりヒト細胞を用いた三次元組織を構築し、これまで生体では解析困難であった組織内リンパ管のネットワーク形成における細胞動態とそれを制御する分子機構の一端を明らかにした。本研究システムは実験動物を用いずにヒト生体組織における細胞動態や分子機構を解析できる新しい実験動物代替研究ツールであると同時に再生医療材料開発や創薬応用モデルとして高い有用性を発揮する。
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