2017 Fiscal Year Research-status Report
胃癌におけるCCND1, CCNE1及びCDK6 遺伝子増幅の検索
| Project/Area Number |
16K08686
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| Research Institution | Kanazawa University |
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Principal Investigator |
大井 章史 金沢大学, 医学系, 教授 (50160411)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | gene amplification / breast cancer / FISH / MLPA |
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| Outline of Annual Research Achievements |
An archive of 322 formalin-fixed and paraffin-embedded invasive ductal cancer tissues were screened by multiple ligation-dependent probe amplification and, a total 885 gene loci judged as ‘gain’ or ‘amplified’ concerning the panel of the 22 genes was further examined for the respective gene amplification by fluorescence in situ hybridization. The results showed 111 of 322 tumors (35%) displayed gene amplification of at least one of the 22 genes. The frequencies of the amplification of four regions with known driver oncogenes were as follows: 8p11 (ZNF703, FGFR1, ADAM9 and IKBKB), 8q24 (MTDH, MYC), 11q13 (CCND1, C11ORF30), and 17q11-21 (CPD, MED1, ERBB2, CDC6, TOP2A, MAPT) were 10%, 12.7%, 12.4%, and 12.7%, respectively. In addition to homogeneously staining region-type or double minute chromosome-type amplifications, the centromere-associated type amplification in nine tumors. Co-localization of the amplicon on 8p11 and the amplicon on 11q13 in single cells was found in 10 tumors, and in the six of them the two amplicons constituted single amplification units. Similarly, amplicon consisting ERBB2 and its franking genes on 7q12-21 co-localized with the amplicon on 8p11 in 10 tumors and with the amplicon on 11q13 in five tumors.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
遺伝子増幅の検索方法についての方法論を以下の論文で出版した。
Ooi A and Oyama T. Detection of CCND1 gene copy number variations using multiplex ligation-dependent probe amplification and fluorescence in situ hybridization Methods.
Methods Mol Biol. 2018;1726:101-109. doi: 10.1007/978-1-4939-7565-5_10.
PMID: 29468547
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| Strategy for Future Research Activity |
引きつずき胃癌、乳癌について、8p11-12、11q11.3 と17q12-21の増幅をMLPAとFISHを使って解析し、増幅遺伝子を同定し、その意義を解明すると同時に、異なる染色体上の遺伝子が同時増幅するメカニズムを明らかにしたい。
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| Causes of Carryover |
剰余金が出たが研究遂行上問題がなかった。
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