Regulatory mechanism of ciliary protein Inversin by Akt

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K08705
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Hokkaido University
• Principal Investigator: Suizu Futoshi 北海道大学, 遺伝子病制御研究所, 准教授 (90431379)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: Akt / Inversin / 細胞極性 / リン酸化 / 一次繊毛

Outline of Final Research Achievements

The Akt phosphorylation site amino acid sequence Thr-Ser-Thr (864-866) of human Inversin is conserved in higher animals such as primates, but not in animal groups such as mice, frogs and zebrafish. There was no effect on intracellular localization of Inversin or Wnt signal inhibitory activity. Thus, there would be no functional conservation of Inversin phosphorylation by Akt. Moreover, Inversin was found to be accumulated in some nuclear structure which likely to be a transcriptional activation center and has a specific transcription activity. Taken together, this study strongly suggests that Inversin functions as a novel transcriptional regulator.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

本研究成果を足掛かりに、Inversinをはじめとした様々な繊毛タンパク群のこれまでに知られていない転写制御因子としての生物機能発見に繋がると考えている。そして、それらの生物機能の理解により、これまで未解明であった繊毛タンパク異常が引き起こす平面内細胞極性異常や繊毛病発症の分子メカニズム解明の道標になると考えている。