Analyzing PREB, the transcriptional factor, transgenic mouse for treatment of lifestyle disease

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K09786
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Kagawa University
• Principal Investigator: Imachi Hitomi 香川大学, 医学部, 准教授 (80380187)
• Co-Investigator(Kenkyū-buntansha): 村尾 孝児 香川大学, 医学部, 教授 (20291982)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 非アルコール性脂肪肝炎 / 生活習慣病 / PREB / ABCA1

Outline of Final Research Achievements

Previously, we reported that the transcriptional factor, prolactin regulatory element binding (PREB) relates with lifestyle disease, such as diabetes mellitus and atherosclerosis. That is, we showed that PREB acts as anti-diabetic in pancreatic β cells and regulates adiponectin expression in adipocytes. In this study, we tried to clear the role of PREB on lifestyle disease. Results are; (1) ABCA1 is a pivotal regulator of lipid efflux from cells, and PREB increases ABCA1 expression in hepatocyte by binding to ABCA1 promoter. As a result, PREB improve fatty liver. (2) PREB regulates adiponectin expression in vivo. (3)PREB may effect protects pancreatic β cells improving lipotoxicity via ABCA expression.
Thus ABCA1 should be the target molecule of PREB, and we need to elucidate the mechanism of ABCA1 expression by PREB for lifestyle disease treatment.

Free Research Field

内分泌代謝学

Academic Significance and Societal Importance of the Research Achievements

転写因子PREBがABCA1分子発現調節を通じて細胞内脂肪蓄積やそれによる細胞機能障害により生活習慣病に関連していることが判明した。今後さらにPREBによるABCA1遺伝子の発現調節が新たな生活習慣病の治療方法に繋がると考えられる。