2016 Fiscal Year Research-status Report
PNHの真の病態とは何か？：PNHにおける網羅的遺伝子解析とiPS細胞バンク創設
| Project/Area Number |
16K09854
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
野地 秀義 福島県立医科大学, 医学部, 准教授 (20347214)
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| Co-Investigator(Kenkyū-buntansha) |
七島 勉 福島県立医科大学, 医学部, 博士研究員 (10192105)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | PNH / iPS細胞 / DNA解析 |
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| Outline of Annual Research Achievements |
我々は、これまでの研究と東北6県および北海道地区のPNH症例(登録50例)を対象とした凝固異常、造血状態異常および自己免疫異常の病態調査を行っており、PNHでこれまで考えられていた溶血以外の因子による血栓発症機序、PNHクローンの拡大には、腫瘍性増殖や免疫学的な攻撃を回避する機序、免疫学的異常や造血障害による血球減少が複雑に絡み合っている所見を得た。
本研究では、臨床病態が明らかなPNH症例50例を対象として、網羅的遺伝子解析を行いPNHの遺伝子異常を明らかにするとともに、種々の病態を示すPNH症例由来のiPS細胞を作成し、PNH病態モデルを作成する事を目的とする。
本年度は、本研究のiPS細胞の実験に関しては、倫理審査の審査、実験器具・施設の準備にとどまった。現在、健常人細胞を用いた予備実験を開始している。
DNA解析は、順次、提出している。
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| Current Status of Research Progress |
Current Status of Research Progress
4: Progress in research has been delayed.
Reason
所属の変更に伴い、これまでの人員の確保などができなかったため、iPS細胞の作製は、著しく進行が遅れた。
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| Strategy for Future Research Activity |
iPS細胞の作成は、昨年度行うべき内容を本年度実行する。健常人での予備実験は進行しているので、昨年度の本来の進行よりは進行度は早いと思われる。
DNA解析は、症例の蓄積を急ぐ。
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| Causes of Carryover |
研究が進まず、残金が多額に生じたため
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| Expenditure Plan for Carryover Budget |
前年度の計画を早急に進める。
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[Journal Article] Clinicopathological characteristics of malignant lymphoma in patients with hepatitis C virus infection in the Tohoku district in Eastern Japan.2017
Author(s)
Tajima K, Takahashi N, Ishizawa K, Murai K, Akagi T, Noji H, Sasaki O, Wano M, Itoh J, Kato Y, Shichishima T, Harigae H, Ishida Y; Tohoku Hematology Forum.
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Journal Title
Leuk Lymphoma.
Volume: 58(6)
Pages: 1509-1511
DOI
Peer Reviewed
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[Journal Article] Evaluation of the safety and efficacy of recombinant soluble thrombomodulin for patients with disseminated intravascular coagulation associated with acute leukemia: multicenter prospective study by the Tohoku Hematology Forum.2017
Author(s)
Yokoyama H, Takahashi N, Katsuoka Y, Inomata M, Ito T, Meguro K, Kameoka Y, Tsumanuma R, Murai K, Noji H, Ishizawa K, Ito S, Onishi Y, Harigae H; Tohoku Hematology Forum.
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Journal Title
International Journal of Hematology
Volume: 105(5)
Pages: 606-613
DOI
Peer Reviewed
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[Journal Article] High prevalence of diffuse large B-cell lymphoma in occult hepatitis B virus-infected patients in the Tohoku district in Eastern Japan2016
Author(s)
Katsushi Tajima, Naoto Takahashi, Kenichi Ishizawa, Kazunori Murai, Tomoaki Akagi, Hideyoshi Noji, Osamu Sasaki, Masaharu Wano, Jugoh Itoh, Yuichi Kato, Tsutomu Shichishima, Yoji Ishida, Hideo Harigae, Kenichi Sawada, The Tohoku Hematology Forum
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Journal Title
Journal of Medical Virology
Volume: 88 (12)
Pages: 2206 - 2210
DOI
Peer Reviewed / Open Access
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[Journal Article] Causes of macrocytic anemia among 628 patients: mean corpuscular volumes of 114 and 130 fL as critical markers for categorization2016
Author(s)
Natsuko Takahashi, Junichi Kameoka, Naoto Takahashi, Yoshiko Tamai, Kazunori Murai, Riko Honma, Hideyoshi Noji, Hisayuki Yokoyama, Yasuo Tomiya, Yuichi Kato, Kenichi Ishizawa, Shigeki Ito, Yoji Ishida, Kenichi Sawada, Hideo Harigae
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Journal Title
International Journal of Hematology
Volume: 10 (3)
Pages: 344 - 357
DOI
Peer Reviewed
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[Journal Article] Analysis of chromosome translocation frequency after a single CT scan in adults2016
Author(s)
Y Abe, T Miura, MA Yoshida, R Ujiie, Y Kurosu, N Kato, A Katafuchi, N Tsuyama, F Kawamura, T Ohba, T Inamasu, F Shishido, H Noji, K Ogawa, H Yokouchi, K Kanazawa, T Ishida, S Muto, J Ohsugi, H Suzuki, T Ishikawa, K Kamiya, A Sakai
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Journal Title
Journal of Radiation Research
Volume: 57 (3)
Pages: 220 - 226
DOI
Peer Reviewed
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[Journal Article] Reduction of c-Fos via overexpression of miR-34a results in enhancement of TNF-α production by LPS in neutrophils from myelodysplastic syndrome patients2016
Author(s)
Yayoi Shikama, Meiwan Cao, Tomoyuki Ono, Xiaomin Feng, Hideyoshi Noji, Hideo Kimura, Kazuei Ogawa, Yuko Suzuki, Kazuhiko Ikeda, Yasuchika Takeishi, Junko Kimura
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Journal Title
PLOS ONE
Volume: 11 (18)
Pages: e0158527
DOI
Peer Reviewed / Open Access
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[Presentation] The first follow-up data analysis of patients with acquired bone marrow failure harboring a small population of PNH-Type cells in the Japanese, Multicenter, Prospective Study Optima2016
Author(s)
Yukari Shirasugi, Chiharu Sugimori, Hideyoshi Noji, Tsutomu Shichishima, Naoshi Obara, Shigeru Chiba, Haruhiko Ninomiya, Yoshihiko Nakamura, Kiyoshi Ando, Yuji Yonemura, Tatsuya Kawaguchi, Kohei Hosokawa, Yasutaka Ueda, Jun-ichi Nishimura, Yuzuru Kanakura, Shinji Nakao.
Organizer
58th American Society of Hematology (ASH) Annual Meeting and Exposition
Place of Presentation
San Diego, USA
Year and Date
2016-12-03 – 2016-12-06
Int'l Joint Research
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[Presentation] Relationship between thrombosis and coagulation markers in patients with PNH.2016
Author(s)
H Noji, K Murai, J Kameoka, S Ito, O Yasushi, K Takada, S Saito, H Kimura, K Ikeda, K Yamaguchi, N Fujishima, H Yokoyama, H Kanbayashi, J Kato, N Takahashi, O Eijirou, K Ishizawa, Y Ishida, H Harigae, Y Takeishi, T Shichishima.
Organizer
第78回 日本血液学会学術集会
Place of Presentation
横浜
Year and Date
2016-10-13 – 2016-10-15
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[Presentation] Two-year consolidation by nilotinib might improve the TFR of STIM trial in CML with DMR.2016
Author(s)
Kaichi Nishiwaki, Naoto Takahashi, Chiaki Nakaseko, Hisashi Wakita, Koji Sano, Chikako Ohwada, Nobuyuki Aotsuka, Jun Kuroki, Hideo Kimura, Hideyoshi Noji, Michihide Tokuhira, Kinuko Mitani, Kazuhisa Fujikawa, Osamu Iwase, Kohshi Ohishi, Fumihiko Kimura, Tetsuya Fukuda, Sakae Tanosaki, Masatomo Miura, Kenichi Sawada.
Organizer
第78回 日本血液学会学術集会
Place of Presentation
横浜
Year and Date
2016-10-13 – 2016-10-15
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[Presentation] High sensitivity flow cytometry to detect small population of PNH clone in bone marrow failure syndrome in Japan.2016
Author(s)
Yasutaka Ueda, Jun-ichi Nishimura, Chiharu Sugimori, Kohei Hosokawa, Yuji Yonemura, Naoshi Obara, Hideyoshi Noji, Yoshihiko Nakamura, Yukari Shirasugi, Kiyoshi Ando, Tsutomu Shichishima, Haruhiko Ninomiya, Shigeru Chiba, Tatsuya Kawaguchi, Yuzuru Kanakura, Shinji Nakao.
Organizer
26th International Complement Workshop
Place of Presentation
Kanazawa, Japan
Year and Date
2016-09-04 – 2016-09-08
Int'l Joint Research
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[Presentation] A clinical significance and time-dependent change of PHN clone size in patients with bone marrow failure syndrome: Japanese Mmulti-centre prospective study.2016
Author(s)
Yukari Shirasugi, Chiharu Sugimori, Hideyoshi Noji, Tsutomu Shichishima, Naoshi Obara, Shigeru Chiba, Haruhiko Ninomiya, Yoshihiko Nakamura, Kiyoshi Ando, Yuji Yonemura, Tatsuya Kawaguchi, Kohei Hosokawa, Yasutaka Ueda, Jun-ichi Nishimura, Yuzuru Kanakura, Shinji Nakao.
Organizer
21st Congress of European Hematology Association
Place of Presentation
Copenhagen, Denmark
Year and Date
2016-06-09 – 2016-06-12
Int'l Joint Research
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