2018 Fiscal Year Final Research Report

Functional analysis of iPS cell-derived cardiomyocytes with hereditary cardiomyopathy

Research Project

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Project/Area Number	16K10080
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Pediatrics
Research Institution	Nippon Medical School
Principal Investigator	YASUHIRO KATSUBE 日本医科大学, 医学部, 准教授 (20246523)
Co-Investigator(Kenkyū-buntansha)	赤尾見春日本医科大学, 医学部, 助教 (60350112) 星野レイ日本医科大学, 医学部, 助教 (20637821) 上田美希日本医科大学, 医学部, 助教 (20741010)
Co-Investigator(Renkei-kenkyūsha)	OGAWA shunichi 日本医科大学, 医学部, 教授 (50194436) NAKANISHI toshio 東京女子医科大学, 医学部, 教授 (90120013) HAYAMA emiko 東京女子医科大学, 医学部, 助教 (00349698)
Project Period (FY)	2016-04-01 – 2019-03-31
Keywords	iPS細胞 / 遺伝性心筋症 / 心筋細胞 / パッチクランプ法 / 電気整理
Outline of Final Research Achievements	The purpose of this study was to create iPS-derived cardiomyocytes from patients with hereditary cardiomyopathy and to investigate the electrophysiological characteristics of the generated iPS cell-derived cardiomyocytes. We were able to prepare iPS-derived cardiomyocytes from healthy subjects and patients with dilated cardiomyopathy (DCM) (1-year-old girl). The electrophysiology study could be carried out in prepared cardiomyocytes using the MED64 systems. It was confirmed that the QT time of DCM patients was prolonged than that of healthy individuals, and it was shortened by β stimulant, isoproterenol. Future evaluations using other electrophysiology methods will be added. We would like to consider other hereditary cardiomyopathies as well and broaden our research.
Free Research Field	小児循環器
Academic Significance and Societal Importance of the Research Achievements	患者自身の心筋細胞を用いた遺伝性心筋症についての研究、特に細胞レベルから見た機能解析の研究はこれまで全く行われていない。近年これまでヒトを用いた研究が難しかった領域でiPS細胞を用いた研究が盛んにおこなわれるようになり、新しい知見が次々と報告されるに至っている。不整脈領域ではQT延長症候群のへの応用がかなり進んでいるが、遺伝性心筋症の患者iPS細胞を用いた研究はほとんど行われていない。本研究により、遺伝性心筋症患者への心筋細胞レベルでの薬剤評価などが進むものと期待される。