Development of new high resolution chromosome biodosimetry method using cell synclonaization and FISH

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K10337
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Hirosaki University
• Principal Investigator: Yoshida Mitsuaki 弘前大学, 被ばく医療総合研究所, 教授 (60182789)
• Co-Investigator(Kenkyū-buntansha): 三浦 富智 弘前大学, 保健学研究科, 准教授 (20261456) ; 有吉 健太郎 弘前大学, 被ばく医療総合研究所, 助教 (50462750)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 染色体線量評価法 / PCC-ring法 / 細胞同調法

Outline of Final Research Achievements

In this study, we confirmed the usefulness of using the cell synchronization method and the PCC method together in order to establish a highly accurate dose evaluation method corresponding to a wide range of exposure dose. From the previous studies, it has become clear that the 8-hour release over a wide range of 0 to 15 Gy can obtain high-frequency G2 / M phase cells, confirming the effectiveness of the combination of cell synchronization methods. In addition, as a result of analyzing whether or not the frequency of cyclic chromosomes is affected under various conditions, no significant difference was obtained when compared at the same release time. On the other hand, as a result of analyzing by changing the dose at the same release time, dose dependency was confirmed. At this stage, it is considered urgent to clearly define the criteria, as the criteria for circular chromosomes are ambiguous.

Free Research Field

分子細胞遺伝学

Academic Significance and Societal Importance of the Research Achievements

現実の被ばく事故を想定した場合に0～20Gyの広範囲にわたって一つの手法で線量評価が可能となれば、早期に被ばく線量の推定に大きく貢献する。本研究において解析の対象とした環状染色体も線量依存性があることから、細胞同調法を用いて方法が確立することが急務と考えられる。本研究で得られた結果は未だ改良の余地があることが明らかとなった。また、環状染色体の解析基準も国際的に早急に確立することが必要と考えられる。