2018 Fiscal Year Final Research Report
The establishment of molecular testing using cytological materials for lung cancer treatments
| Project/Area Number |
16K10690
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory surgery
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| Research Institution | Kyorin University |
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Principal Investigator |
Tanaka Ryota 杏林大学, 医学部, 准教授 (40415063)
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| Research Collaborator |
Tachibana Keisei
Kamma Hiroshi
Fujiwara Masachika
Kondo Haruhiko
Ohtsuka Kouki
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | 肺癌 / コンパニオン診断 / 気管支鏡 / 液状化検体細胞診 / EGFR / KRAS / ROS-1 / ALK |
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| Outline of Final Research Achievements |
We studied patients with lung cancer undergoing bronchoscopic biopsy to assist us with making treatment plans. After getting cytological samples using instrument washings, the specimen was fixed by BD CytoRich Red Preservative, making a cellblock with iPGell. The cellblocks with iPGell were used for cytomorphological subtyping, molecular testing and immunocytochemical analysis for validation of feasibility in this study. The cellblock with iPGell was suitable for use in these kinds of examinations, and it might be an optimal preparation for analyzing gene aberrations before developing treatment plans in our clinical practices.
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| Free Research Field |
呼吸器外科学、病理・細胞診断学
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| Academic Significance and Societal Importance of the Research Achievements |
非小細胞肺癌の薬物療法は分子標的治療や、免疫療法がめざましい発展を遂げてきた。気管支鏡下生検による確定診断は重要であり、薬剤耐性後の再生検も更に必要なことがある。本研究において微量検体を用いた免疫組織学的な組織型推定、更に分子標的治療薬などの効果予測となる遺伝子解析を高感度に実行した。そして細胞浮遊液ゼリー化試薬（iPGell）を用いた方法の検証実験を行い、本法が簡便かつ効率的な方法であることを証明した。
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