2018 Fiscal Year Annual Research Report
Alcohol-induced mitophagy in testicular cells-a basic study related to male infertility treatment
| Project/Area Number |
16K11073
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| Research Institution | Osaka Medical College |
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Principal Investigator |
Eid NabilA.S. 大阪医科大学, 医学部, 講師 (50570165)
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| Co-Investigator(Kenkyū-buntansha) |
田中 義久 大阪医科大学, 医学部, 助教 (20648703)
伊藤 裕子 大阪医科大学, その他部局等, 功労教授 (40148432)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | Autophagy / Mitophagy / Parkin / Testis / Sertoli / Apoptosis / Ethanol / Infertility |
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| Outline of Annual Research Achievements |
Using various methods such as immunoelectron microscopy and Western blot, IHC and IF methods, I detected that autophagy and PINK1-Parkin mitophagy pathway is upregulated in Sertoli cells (SCs) of acute ethanol-treated rats (ETRs) (5 gm/kg/bw), although many germ cells underwent apoptosis. The upregulation of Parkin in SCs of ETRs, compared to control may be anti-apoptotic mechanism against ethanol damaging effects. This enhanced autophagic response in SCs may be important also for survival of germ cells by supply of essential nutrition via a Warburg like mechanism and phagocytosis of germ cells via autophagy-associated phagocytosis. Stimulation of Parkin-related mitophagy SCs by natural and pharmaceutical methods may help in treatment of infertility associated with alcohol disorders.
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