2018 Fiscal Year Annual Research Report
Clinical significance of MMPs and TIMPs in pathogenesis of patients with recurrent pregnancy loss
| Project/Area Number |
16K11100
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| Research Institution | Nagoya City University |
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Principal Investigator |
尾崎 康彦 名古屋市立大学, 大学院医学研究科, 教授 (50254280)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | 不育症 / 反復流産 / 脱落膜 / 絨毛 / 頸管粘液 / d-ROM / BAP |
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| Outline of Annual Research Achievements |
Increased levels of oxidative stress has been reported with its negative effects in various diseases. We examined the role of oxidative stress in the patients with recurrent pregnancy loss (RPL).
Reactive oxygen metabolites (d-ROMs) and biological anti-oxidant potential levels (BAP) in plasma between non-pregnant RPL patients [n=21 in total, including 10 anti-phospholipid antibody syndrome (APS) patients and 11 non-APS patients] and healthy controls (n=10) were compared. d-ROMs and BAP in the cervical mucus (CM) of the RPL patients (n=30) in their early pregnancy were measured and followed up the pregnancy outcome: a spontaneous miscarriage group (n=20) and a live-birth group (n=10). Decidua and villi were surgically taken under the diagnosis of missed abortion and analyzed d-ROMs and BAP between with and without the fetal chromosome aberration (n=10 each).
d-ROMs (p=0.0005) and BAP (p=0.01) in plasma were significantly higher in the RPL patients than controls. Plasmic d-ROMs and BAP were the highest in the APS patients and the lowest in the controls. A highly significant positive correlation was observed between d-ROMS and BAP in CM (live-birth: r=0.71, p=0.02, miscarriage: r=0.98, p<0.0001). There was no significant difference in villi, but decidua showed the increase of d-ROMs (p=0.06) and BAP (p=0.04) in the normal fetal chromosome group.
We demonstrated for the first time that d-ROMs and BAP are detectable in CM and the product of conception. The imbalance of dROMs and/or BAP plays an important role in pathogenesis of idiopathic RPL and also RPL patients with APS.
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