Investigation of the role of miRNA in syndromic craniosynostosis

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K11779
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KOBAYASHI Yukiho 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (20596233)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 頭蓋骨縫合癒合症

Outline of Final Research Achievements

We detected 511 kinds of miRNA in the calvarial coronal suture of Apert syndrome model mouse at E15.5 by the miRNA array. Significantly higher expression of 19 kinds of miRNA in the calvarial tissue of Apert model mouse as compared with that of wild type. On the other hand, 5 kinds of miRNA was significantly decreased in the calvarial tissue of Apert model mouse as compared with that of wild type. By qPCR analysis, mmu-miR-182-5p、mmu-miR-206-3p were significantly higher in the calvarial tissue of Apert model mouse as compared with that of wild type. These results suggested that mmu-miR-182-5p、mmu-miR-206-3p would have some role in the establishment of craniosynostosis in the Apert syndrome model mouse.

Free Research Field

歯科矯正学

Academic Significance and Societal Importance of the Research Achievements

頭蓋冠縫合部の発生過程におけるmiRNAの発現およびその機能は全く不明であったが、本研究により500種類以上のmiRNAが発生中の頭蓋冠縫合部に発現することが明らかとなり、何らかの役割を果たす可能性が示唆された。これにより、顎顔面領域に形態異常を呈する先天性骨代謝疾患に対し、新たな創薬ターゲットを探索し、新規治療法の開発基盤となることが期待される。