2017 Fiscal Year Final Research Report
Design of cross-talking amplification circuit with orthogonal nucleic acid
| Project/Area Number |
16K12522
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Life / Health / Medical informatics
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Keywords | 直交性 / DNA / RNA / 論理回路 / 人工核酸 / 蛍光増幅 |
|---|
| Outline of Final Research Achievements |
Orthogonality is a key concept that has been broadly interpreted to mean that components or elements do not affect each other or behave independently. Previously, we have developed three artificial nucleic acids, D-aTNA, L-aTNA, SNA, composed of acyclic serinol derivatives as scaffolds. All of them can form remarkably stable homo-duplexes. While D-aTNA cross-pairs neither natural DNA (D-DNA) nor RNA (D-RNA), SNA with achiral scaffold can cross-pair with D-, L-aTNA, D-DNA, and D-RNA. In the present study, we designed a logic gate with D-aTNA that is orthogonal to D-RNA, which can be activated by D-RNA input via SNA as an interface: A seesaw gate with D-aTNA and interface SNA targeting miR21 was designed. Fluorescence could successfully be amplified by D-RNA (miR21) input that triggered D-aTNA seesaw gate via SNA.
|
| Free Research Field |
生物有機化学
|
