Multiple mechanisms of post-replication repair pathway choice by deubiquitinases for ubiquitinated PCNA

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K12594
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Nagoya University
• Principal Investigator: MASUDA Yuji 名古屋大学, 医学系研究科(環医), 准教授 (30273866)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 損傷トレランス

Outline of Final Research Achievements

The DNA damage tolerance pathways as the post-replication repair are regulated by ubiquitination of proliferating cell nuclear antigen (PCNA). Since mono- and poly-ubiquitination of PCNA stimulates the error-prone pathway, translesion DNA synthesis (TLS), and the error-free, in principle, pathway, template switch (TS), respectively. However, in humans, the regulatory mechanism is obscure because poly-ubiquitinated PCNA is only slightly detectable. In this study, we identified deubiquitinases for ubiquitinated PCNA and analyzed their functions in the damage tolerance pathways.

Free Research Field

生化学