Direct measurement of interaction force acting on living microbial cells using atomic force microscope

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K12621
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental engineering and reduction of environmental burden
• Research Institution: Osaka Prefecture University
• Principal Investigator: Nomura Toshiyuki 大阪府立大学, 工学(系)研究科(研究院), 准教授 (00285305)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 原子間力顕微鏡 / 細胞 / ナノ粒子 / 相互作用

Outline of Final Research Achievements

In this study, in order to directly measure the interaction force acting on living microbial cells using an atomic force microscope (AFM), we investigated immobilization of one microbial cell or one nanoparticle at the tip of the AFM cantilever. As a result, we demonstrated that a single cell probe on which one living cell is immobilized or a single nanoparticle probe on which one nanoparticle is immobilized could be prepared if the change in free energy when a cell or a nanoparticle adheres to the AFM cantilever is negative.

Free Research Field

粒子－細胞間に働く相互作用力の評価と応用